The EUCAST Antifungal Susceptibility Testing 7 (AFST) subcommittee, formed in 2002, was initially chaired by Juan-Luis Rodriguez-Tudela and, with Peter Donnelly as the scientific secretary, rapidly created a framework for setting breakpoints for both existing and new antifungal agents for both Candida and Aspergillus species.

Committee to harmonize antimicrobial breakpoints, organized by ESCMID, ECDC and European national breakpoint committees. Subcommittees on antifungal susceptibility testing, susceptibility testing of anaerobes and interpretative reading and expert rules in susceptibility testing.

For archived a documents pertaining to antifungals, go to the AFST archive. Breakpoint tables. Clinical breakpoints - bacteria (v 10.0) - pdf for printing (1 Jan - 31 Dec, 2020). Erratum: In the 2020 breakpoint table, imipenem-relebactam breakpoints under Enterobacterales, should read "valid except for Morganellaceae ". The European Committee on Antimicrobial Susceptibility Testing - EUCAST. EUCAST is a standing committee jointly organized by ESCMID, ECDC and European national breakpoint committees.

Candida and Aspergillus infections have emerged as significant pathogens in recent decades. During this same time, broad spectrum triazole and echinocandin antifungal agents have been developed and increasingly used. EUCAST deals with breakpoints and technical aspects of phenotypic in vitro antimicrobial susceptibility testing and functions as the breakpoint committee of EMA and ECDC. EUCAST does not deal with antibiotic policies, surveillance or containment of resistance or infection control. The Steering Committee is the decision making body. Two organisations have developed reference methods for the testing of fungal isolates and associated The subcommittee on antifungal susceptibility testing of the European Committee on Antibiotic Susceptibility Testing (EUCAST) has developed standards for susceptibility testing of fermentative Two organisations have developed reference methods for the testing of fungal isolates and associated Breakpoints for antifungal agents: An update from EUCAST focussing on echinocandins against Candida spp.

Committee to harmonize antimicrobial breakpoints, organized by ESCMID, ECDC and European national breakpoint committees. Subcommittees on antifungal susceptibility testing, susceptibility testing of anaerobes and interpretative reading and expert rules in susceptibility testing.

Objectives: To present an overview of the current antifungal breakpoints and supporting evidence behind the changes. 2013-06-01 for Disease Control and Prevention (ECDC), and European national breakpoint commit-tees, EUCAST harmonized standards for countries both within and outside Europe. In 1997, the EUCAST antifungal susceptibility testing subcommittee formed, and their ﬁrst published standard of susceptibility testing for both yeasts and molds was released in 2008 Despite the importance of antimicrobial susceptibility testing (AST) for clinical management of infection and antimicrobial resistance (AMR) surveillance, the methodologies and breakpoints of the two most commonly used systems worldwide, Clinical and Laboratory Standards Institute (CLSI) and European Committee for Antimicrobial Susceptibility Testing (EUCAST), are far from harmonized. Antifungal Agents Breakpoint tables for interpretation of MICs Version 6.1, valid from 2013-03-11 Notes 1.

Rationale Documents summarize the data on which EUCAST breakpoints for antifungal agents are listed elsewhere, see Antifungal rationale documents".

2. Epidemiology and Patterns of Antifungal Susceptibility of Species Causing Aspergillosis Antifungal breakpoints and susceptibility testing (AFST Subcommittee) Maiken Cavling Arendrup Chairman of EUCAST AFST Prof, MD, PhD, DMSci Statens Serum Institut A brief history of antifungal susceptibility testing standardization 1982 Established subcommittee 1986 Develop reproducible method 1992 M27-P method introduced 1997 M27-A method introduced 20% hospitals performing testing for yeast; intra/inter-laboratory agreement poor Synthetic medium (RPMI) Broth-based method 0.5-2.5 x103 EUCAST Higher glucose; EUCAST technical note on Aspergillus and amphotericin B, itraconazole, and posaconazole. Clin Microbiol Infect. 2012;18(7):E248–50. doi: 10.1111/j.1469-0691.2012.03890.x. Epub 2012 Apr 30.

• Guidance on Stenotrophomons maltophilia 2012 • Guidance on direct AST 2012 • Guidance on systemic breakpoints for oral cephalosporins • Validation of EUCAST zone diameter breakpoints ( many) 2012 and compared their antifungal susceptibility according to clinical breakpoints deﬁned by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) in 2013, and the Clinical and Laboratory Standards Institute (CLSI) in 2008 (old CLSI breakpoints) and 2012 Clin Microbiol Infect 2011; 17: E27–E29 Abstract The European Committee on Antimicrobial Susceptibility Testing‐Subcommittee on Antifungal Susceptibility Testing (EUCAST‐AFST) has determined breakp 2013-01-15 Breakpoints for previously established antibacterial and antifungal agents in Europe have now been harmonized. With the EMA, EUCAST has since 2006 determined breakpoints for new agents. All breakpoints are freely available on the EUCAST web site; these are used in semi-automated antimicrobial susceptibility testing devices and have been employed since 2010 in a EUCAST disc diffusion method.
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EUCAST Definitive Document EDef7.1:method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts. The role of antimicrobial susceptibility testing is to aid in selecting the best agent for the treatment of bacterial and fungal diseases. This has been best achieved by the setting of breakpoints by Clinical Laboratory Standards Institute (CLSI) for prevalent Candida spp. versus anidulafungin, caspofungin, micafungin, fluconazole, and voriconazole.

EUCAST was formed in 1997. It has been chaired by Ian Phillips (1997 - 2001), Gunnar Kahlmeter (2001 - 2012), Rafael Canton 2012 - 2016) and Christian Giske (2016 - ).
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(EUCAST-AFST) has reviewe d all current antifungal breakpoints and recently released a revised breakpoint table, v 1 0.0 BPs, and eight revised rationale documents.

versus anidulafungin, caspofungin, micafungin, fluconazole, and voriconazole. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) also has set breakpoints for prevalent and common Candida and Aspergillus species versus amphotericin B, itraconazole, and posaconazole. Committee to harmonize antimicrobial breakpoints, organized by ESCMID, ECDC and European national breakpoint committees.